Antigen

Antigen Class

TFs and others

Antigen

HMGB1

Cell type

Cell type Class

Lung

Cell type

IMR-90

Primary Tissue

Lung

Tissue Diagnosis

Normal

Sample

source_name

Normal lung fibroblast IMR90

cell line

IMR90

condition

Proliferating

chip antibody

HMGB1 Hybridoma Ab (PCRP-HMGB1-4F10)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

~25 million cells per sample were double crosslinked in EGS/PFA and processed with ChIP-IT high sensitivity kit (Active motif) as per manufacturer instructions. Immunoprecipitation was carried out with 4 ug of HMGB1 antibody (Hybridoma, PCRP-HMGB1-4F10) in 30 ug of chromatin. DNA was purified with ChIP DNA Clean & Concentrator kit (Zymo). TruSeq Library Preparation Kit (Illumina).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

34159865

Reads aligned (%)

98.1

Duplicates removed (%)

6.6

Number of peaks

1595 (qval < 1E-05)

hg19

Number of total reads

34159865

Reads aligned (%)

97.8

Duplicates removed (%)

7.4

Number of peaks

1589 (qval < 1E-05)